Difference between revisions of "Part:BBa J45006:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45006 short</partinfo>
 
<partinfo>BBa_J45006 short</partinfo>
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===Design Notes===
 
===Design Notes===
 +
 
*Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G
 
*Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G
 
*forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA TGA AAT CGA TGA G</b> -3'
 
*forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA TGA AAT CGA TGA G</b> -3'
 
*reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA<b>T TAT TAA GGG CCT AAA AGG</b> -3'
 
*reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA<b>T TAT TAA GGG CCT AAA AGG</b> -3'
 +
 +
<b>WE WANT TO REORDER: new forward and new reverse primers (6/26) </b>
 +
 +
*new forward primer: 5' - GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA TGA AAT CGA TGA GAA AAA TC</b> -3'
 +
*new reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT <b>TAa ggg cct aaa agg aga gct ttg</b> -3'
 +
 
*forward mutagenesis primer: 5' GAA GCA AAT ATT AGA AGA GTT CAA AAA TAG TAA GGG 3'
 
*forward mutagenesis primer: 5' GAA GCA AAT ATT AGA AGA GTT CAA AAA TAG TAA GGG 3'
−
*reverse mutagenesis primer: 5' CCCTTACTATTTTTGAACTCTTCTAATATTTGCTTC 3'
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*reverse mutagenesis primer: 5' CCC TTA CTA TTT TTG AAC TCT TCT AAT ATT TGC TTC 3'
  
 
===Source===
 
===Source===
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===References===
 
===References===
 
<biblio>
 
<biblio>
−
#*Horton03 pmid=12937998
+
#Horton03 pmid=12937998
 +
#Singh--2007 pmid=17891501
 
</biblio>
 
</biblio>

Latest revision as of 15:48, 5 February 2008

alcohol acetyltransferase I (ATF1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


Design Notes

  • Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G
  • forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA TGA AAT CGA TGA G -3'
  • reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG -3'

WE WANT TO REORDER: new forward and new reverse primers (6/26)

  • new forward primer: 5' - GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA TGA AAT CGA TGA GAA AAA TC -3'
  • new reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAa ggg cct aaa agg aga gct ttg -3'
  • forward mutagenesis primer: 5' GAA GCA AAT ATT AGA AGA GTT CAA AAA TAG TAA GGG 3'
  • reverse mutagenesis primer: 5' CCC TTA CTA TTT TTG AAC TCT TCT AAT ATT TGC TTC 3'

Source

Source: S. cerevisiae, chromosome XV

Sequence obtained from Genbank, accession number [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285]

References

<biblio>

  1. Horton03 pmid=12937998
  2. Singh--2007 pmid=17891501

</biblio>