Difference between revisions of "Part:BBa J45005:Design"

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===Source===
 
===Source===
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#GenBank Accession No. AF133053
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#GenBank Accession No. [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6002711 AF133053]
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#Species: Clarkia Breweri
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#Species: ''Clarkia breweri''
  
 
===References===
 
===References===

Revision as of 21:06, 2 February 2008


SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winterg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 652
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 232


Design Notes

  1. no restriction enzyme cut sites in coding region
  2. changed natural stop codon to TAA TAA

Source

  1. GenBank Accession No. [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6002711 AF133053]
  2. Species: Clarkia breweri

References

<biblio>

  1. Ross-ArchBiochemBiophys-1999 pmid=10375393

</biblio>

  • Wiki Link: http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6