Difference between revisions of "Part:BBa J45001:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J45001 short</partinfo> | <partinfo>BBa_J45001 short</partinfo> | ||
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===Source=== | ===Source=== | ||
− | #GenBank Accession No. AF515284 | + | #GenBank Accession No. [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=23957317 AF515284] |
#Species = ''Antirrhinum majus'', Snapdragon | #Species = ''Antirrhinum majus'', Snapdragon | ||
===References=== | ===References=== | ||
− | + | <biblio> | |
− | # | + | #Negre-ArchBiochemBiophys-2002 pmid=12361714 |
− | + | </biblio> | |
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− | + | *Wiki Link http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 | |
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Revision as of 20:38, 2 February 2008
SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 577
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 901
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 421
Illegal NgoMIV site found at 425 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 471
Illegal SapI.rc site found at 75
Design Notes
WE WANT TO REORDER: the reverse and the internal sequencing primers (6/26)
- no restriction enzyme cut sites in coding region
- changed natural stop codon to TAA TAA
- primer design
- forward = 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAC AAA ACA AAC ACA AAA G -3'
- reverse = 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAc tct cgc ctt gtc atg gaa ata g -3'
- internal sequencing primer = 5' - gga atg ctg cta tat ttg gg
Source
- GenBank Accession No. [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=23957317 AF515284]
- Species = Antirrhinum majus, Snapdragon
References
<biblio>
- Negre-ArchBiochemBiophys-2002 pmid=12361714
</biblio>
- Wiki Link http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6