Difference between revisions of "Part:BBa J45004:Design"

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===Source===
 
===Source===
  
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Species: ''Petunia x hybrida'' from GenBank (AY233465); (also available: ''A. thaliana'' and ''N. suaveolens'')
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Species: ''Petunia x hybrida'' from GenBank ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465]); (also available: ''A. thaliana'' and ''N. suaveolens'')
  
 
===References===
 
===References===

Revision as of 20:34, 2 February 2008

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Just CDS taken from GenBank

Designed Forward Strand (1 and 2): 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'

  • LENGTH: 19
  • GC CONTENT: 36.8 %
  • MELT TEMP: 47.8 ºC


Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

  • LENGTH: 22
  • GC CONTENT: 27.3 %
  • MELT TEMP: 46.6 ºC


Source

Species: Petunia x hybrida from GenBank ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465]); (also available: A. thaliana and N. suaveolens)

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>