Difference between revisions of "Part:BBa K2810001"
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The [https://en.wikipedia.org/wiki/GUS_reporter_system GUS reporter gene system] is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry. | The [https://en.wikipedia.org/wiki/GUS_reporter_system GUS reporter gene system] is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry. | ||
| − | The GUS gene is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 | + | The GUS gene is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 BBa_P10500]) using BsmBI/Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. |
This has been used to generate the composite reporter constructs detailed in the [http://2018.igem.org/Team:Cardiff_Wales/Composite_Part 2018 Cardiff_Wales Composite Parts page]. | This has been used to generate the composite reporter constructs detailed in the [http://2018.igem.org/Team:Cardiff_Wales/Composite_Part 2018 Cardiff_Wales Composite Parts page]. | ||
| − | + | Using this part, the Cardiff iGEM team of 2018 characterised [https://parts.igem.org/Part:BBa_P10101 35S CaMV promoter], the [https://parts.igem.org/Part:BBa_K2810002 RTBV promoter], [https://parts.igem.org/Part:BBa_P10401 Nopaline synthase terminator], [https://parts.igem.org/Part:BBa_P10400 35S CaMV terminator], and [https://parts.igem.org/Part:BBa_P10402 G7 terminator]. | |
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Latest revision as of 22:12, 4 October 2018
GUS reporter for plant expression
The GUS reporter gene system is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry.
The GUS gene is cloned into the golden gate version of pSB1C3 (BBa_P10500) using BsmBI/Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.
This has been used to generate the composite reporter constructs detailed in the [http://2018.igem.org/Team:Cardiff_Wales/Composite_Part 2018 Cardiff_Wales Composite Parts page].
Using this part, the Cardiff iGEM team of 2018 characterised 35S CaMV promoter, the RTBV promoter, Nopaline synthase terminator, 35S CaMV terminator, and G7 terminator.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]