Difference between revisions of "Part:BBa K2842666"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2842666 short</partinfo>
 
<partinfo>BBa_K2842666 short</partinfo>
 +
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 +
! colspan="2" style="background:#FFBF00;"|Flexible Cloning System
 +
|-
 +
|'''Function'''
 +
|Standardised blue/white measurement
 +
|-
 +
|'''Use in'''
 +
|E. coli cells
 +
|-
 +
|'''Chassis Tested'''
 +
|DH5α cells
 +
|-
 +
|'''Abstraction Hierarchy'''
 +
|Composite Part
 +
|-
 +
|'''Related Device'''
 +
|[https://parts.igem.org/Part:BBa_I732902 BBa_I732902]
 +
|-
 +
|'''RFC standard'''
 +
|[https://parts.igem.org/Help:Assembly_standard_10 RFC10],[https://parts.igem.org/Help:Assembly_standard_12 RFC12],[https://parts.igem.org/Help:Assembly_standard_21 RFC21],[https://parts.igem.org/Help:Assembly_standard_23 RFC23] & [https://parts.igem.org/Help:Assembly_standard_25 RFC25] compatible
 +
|-
 +
|'''Backbone'''
 +
|pSB1C3<br>
 +
|-
 +
|'''Submitted by'''
 +
|[http://2018.igem.org/Team:UCL UCL iGEM 2018]
 +
|}
 +
 +
https://static.igem.org/mediawiki/2018/8/8c/T--UCL--FB_lacZ_gel.png
 +
 +
Figure 1 - Final Brick and lacZ cloning comparison
  
 
-
 
-

Revision as of 17:52, 4 October 2018


Golden Gate compatible system with blue-white screening

Flexible Cloning System
Function Standardised blue/white measurement
Use in E. coli cells
Chassis Tested DH5α cells
Abstraction Hierarchy Composite Part
Related Device BBa_I732902
RFC standard RFC10,RFC12,RFC21,RFC23 & RFC25 compatible
Backbone pSB1C3
Submitted by [http://2018.igem.org/Team:UCL UCL iGEM 2018]

T--UCL--FB_lacZ_gel.png

Figure 1 - Final Brick and lacZ cloning comparison

-

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 647
    Illegal BsaI.rc site found at 28