Difference between revisions of "Help:Plasmid backbones/Glossary"
Line 18: Line 18:
 
|colspan=2|
 
|colspan=2|
 
*An example of a BioBrick plasmid without a part insert is '''[[Part:pSB1A3|pSB1A3]]'''.  You can find more on plasmid naming [[Help:Plasmids/Nomenclature|here]]
 
*An example of a BioBrick plasmid without a part insert is '''[[Part:pSB1A3|pSB1A3]]'''.  You can find more on plasmid naming [[Help:Plasmids/Nomenclature|here]]
−
*To find a plasmid part using the "jump to part" sidebar function, erase the default "BBa_" prefix, and enter only the [[Help:Plasmids/Nomenclature|plasmid part name]] (''e.g.,'' psBxyz).
+
*To find a plasmid part using the "jump to part" sidebar function, erase the default "BBa_" prefix, and enter only the [[Help:Plasmids/Nomenclature|plasmid part name]] (''e.g.,'' pSBxyz).
 
|-
 
|-
 
|colspan=2|
 
|colspan=2|
Line 28: Line 28:
 
|
 
|
 
If you just want one of the BioBrick plasmids, it is probably available in the iGEM DNA distribution with the part
 
If you just want one of the BioBrick plasmids, it is probably available in the iGEM DNA distribution with the part
−
[[Part:BBa_P1010| BBa_P1010]] in the cloning site. You will need the cell strain DB3.1 since [[Part:BBa_P1010| BBa_P1010]] (CcdB) is toxic to most strains.
+
[[Part:BBa_P1010| BBa_P1010]], [[Part:BBa_I52001| BBa_I52001]] or [[Part:BBa_I52002| BBa_I52002]] in the cloning site. You will need the cell strain [[Part:BBa_V1005|DB3.1]] since each of these three parts constitutively expresses the protein CcdB which is toxic to most strains.
 
|-
 
|-
 
|colspan=2|
 
|colspan=2|
−
When making a brand new BioBrick part, you will need to choose an appropriate BioBrick plasmid (with the right antibiotic selection gene, genome size, etc) for your construction purposes.  For this reason, we've made the ccdB gene biobrick ([[Part:BBa_P1010| BBa_P1010]] ), which causes cell death when intact.  By doing so, the successful insertion of the part disrupts the ccdB gene and permits growth.  Plasmids that were not cut, or re-ligated without insert do not grow.
+
When making a brand new BioBrick part, you will need to choose an appropriate BioBrick plasmid (with the right antibiotic selection gene, copy number etc.) for your construction purposes.  To make construction and assembly of BioBrick parts easier, most BioBrick plasmids include [[Part:BBa_P1010| BBa_P1010]], [[Part:BBa_I52001| BBa_I52001]] or [[Part:BBa_I52002| BBa_I52002]] by default.  Each of these three default inserts include the ''ccdB'' positive selection marker which kills most ''E. coli'' strains.  Successful insertion of a BioBrick part into any of the BioBrick vectors replaces the ''ccdB'' positive selection marker and enables cells to grow and form colonies.  Plasmids that were not cut or re-ligated without insert do not grow.
  
 
*For a more in-depth walkthrough of how to use construction plasmids, check the [[Registry FAQ|FAQs]] or [[Featured Parts:Cell Death|"Cell Death" Featured Parts]] section
 
*For a more in-depth walkthrough of how to use construction plasmids, check the [[Registry FAQ|FAQs]] or [[Featured Parts:Cell Death|"Cell Death" Featured Parts]] section
Line 42: Line 42:
 
https://static.igem.org/mediawiki/parts/2/22/MultiPartsinplasmid.png
 
https://static.igem.org/mediawiki/parts/2/22/MultiPartsinplasmid.png
 
|
 
|
−
This term refers to a plasmid backbone with singular or multiple BioBricks inserted.  An example of this can be seen to the left, where BioBricks BBa_B0034, BBa_C0010 & BBa_B0015 have been inserted on a Amp-resistant plasmid.
+
This term refers to a plasmid backbone with singular or multiple BioBricks inserted.  An example of this can be seen to the left, where BioBrick parts [[Part:BBa_B0034|BBa_B0034]], [[Part:BBa_C0010|BBa_C0010]] & [[Part:BBa_B0015|BBa_B0015]] have been inserted into an ampicillin resistant plasmid.
 
|-
 
|-
 
|colspan=2|
 
|colspan=2|

Revision as of 04:25, 25 January 2008

Here at the Registry, we use the term "plasmid" in three different ways:

Plasmid (backbone)

Plasmid_nobb.png

BioBrick plasmids form the "backbone" of all BioBrick parts. Every BioBrick part is maintained on at least one BioBrick plasmid. Sometimes, a part is maintained on multiple BioBrick plasmid backbones, which vary in antibiotic resistance and copy number.

  • An example of a BioBrick plasmid without a part insert is pSB1A3. You can find more on plasmid naming here
  • To find a plasmid part using the "jump to part" sidebar function, erase the default "BBa_" prefix, and enter only the plasmid part name (e.g., pSBxyz).

Construction Plasmid

Plasmid_const.png

If you just want one of the BioBrick plasmids, it is probably available in the iGEM DNA distribution with the part BBa_P1010, BBa_I52001 or BBa_I52002 in the cloning site. You will need the cell strain DB3.1 since each of these three parts constitutively expresses the protein CcdB which is toxic to most strains.

When making a brand new BioBrick part, you will need to choose an appropriate BioBrick plasmid (with the right antibiotic selection gene, copy number etc.) for your construction purposes. To make construction and assembly of BioBrick parts easier, most BioBrick plasmids include BBa_P1010, BBa_I52001 or BBa_I52002 by default. Each of these three default inserts include the ccdB positive selection marker which kills most E. coli strains. Successful insertion of a BioBrick part into any of the BioBrick vectors replaces the ccdB positive selection marker and enables cells to grow and form colonies. Plasmids that were not cut or re-ligated without insert do not grow.

Plasmid (with any BioBrick)

MultiPartsinplasmid.png

This term refers to a plasmid backbone with singular or multiple BioBricks inserted. An example of this can be seen to the left, where BioBrick parts BBa_B0034, BBa_C0010 & BBa_B0015 have been inserted into an ampicillin resistant plasmid.

Back to 'Plasmids' Overview |Browse plasmid partsPart icon plasmid.png