Difference between revisions of "Part:BBa I50042"

Revision as of 03:29, 25 January 2008

pSC101 origin of replication

BBa_I50042 is a pSC101 origin of replication. BBa_I50042 is the replication origin found in the pSB4* series of BioBrick vectors.

BBa_I50042 yields vectors with an expected copy number of ~5 per cell [http://openwetware.org/wiki/Molecular_Cloning Molecular Cloning].

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_I50042 short</partinfo>
-pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
+[[Part:BBa_I50042|BBa_I50042]] is a pSC101 origin of replication.  [[Part:BBa_I50042|BBa_I50042]] is the replication origin found in the pSB4* series of BioBrick vectors.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[Part:BBa_I50042|BBa_I50042]] yields vectors with an expected copy number of ~5 per cell [http://openwetware.org/wiki/Molecular_Cloning Molecular Cloning].
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