Difference between revisions of "Part:BBa K2623014"
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This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.<br> | This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.<br> | ||
CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter <partinfo>BBa_J23100</partinfo> into the CAHS circuit to express Lac I <partinfo>BBa_S0100</partinfo> in order to inhabit the initiation of the Lac O <partinfo>BBa_R0011</partinfo> promoter which can only be activated and then expressing CAHS and mRFP <partinfo>BBa_J04650</partinfo> after IPTG is added.<br> | CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter <partinfo>BBa_J23100</partinfo> into the CAHS circuit to express Lac I <partinfo>BBa_S0100</partinfo> in order to inhabit the initiation of the Lac O <partinfo>BBa_R0011</partinfo> promoter which can only be activated and then expressing CAHS and mRFP <partinfo>BBa_J04650</partinfo> after IPTG is added.<br> | ||
| − | The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp. | + | The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp.The plasmid skeleton is pSB1C3. |
https://static.igem.org/mediawiki/parts/4/4e/CAHS_Fig1.png | https://static.igem.org/mediawiki/parts/4/4e/CAHS_Fig1.png | ||
<br> | <br> | ||
| − | + | ===Identification=== | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 08:41, 3 October 2018
Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator)
SUMMARY
This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.
CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter BBa_J23100 into the CAHS circuit to express Lac I BBa_S0100 in order to inhabit the initiation of the Lac O BBa_R0011 promoter which can only be activated and then expressing CAHS and mRFP BBa_J04650 after IPTG is added.
The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp.The plasmid skeleton is pSB1C3.
Identification
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 375
Illegal NheI site found at 1679
Illegal NheI site found at 1702 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2880
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1376
Illegal AgeI site found at 1488 - 1000COMPATIBLE WITH RFC[1000]