Difference between revisions of "Part:BBa I50022"

 
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<partinfo>BBa_I50022 short</partinfo>
 
<partinfo>BBa_I50022 short</partinfo>
  
High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sitesLacks mutations that render BBa_I50020 nonfunctional as a replication origin.
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[[Image:BBa I50022.png|450px]]
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[[Part:BBa_I50022|BBa_I50022]] is a minimal high-copy replication origin derived from pUC19Any vector containing [[Part:BBa_I50022|BBa_I50022]] will be propagated at high copy.
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Revision as of 23:00, 24 January 2008

Minimal pUC19-derived high copy replication origin

BBa I50022.png

BBa_I50022 is a minimal high-copy replication origin derived from pUC19. Any vector containing BBa_I50022 will be propagated at high copy.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]