Difference between revisions of "Part:BBa K2623014"
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===SUMMARY===
 
===SUMMARY===
 
This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.<br>
 
This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.<br>
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CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter(BBa_J23100) into the CAHS circuit to express Lac I(BBa_S0100) in order to inhabit the initiation of the Lac O(BBa_R0011) promoter which can only be activated and then expressing CAHS and mRFP(BBa_J04650) after IPTG is added.<br>
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CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter<partinfo>BBa_J23100</partinfo> into the CAHS circuit to express Lac I<partinfo>BBa_S0100</partinfo> in order to inhabit the initiation of the Lac O<partinfo>BBa_R0011</partinfo> promoter which can only be activated and then expressing CAHS and mRFP<partinfo>BBa_J04650</partinfo> after IPTG is added.<br>
 
The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp.
 
The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp.
  

Revision as of 14:51, 1 October 2018


Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator)

SUMMARY

This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.
CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoterBBa_J23100 into the CAHS circuit to express Lac IBBa_S0100 in order to inhabit the initiation of the Lac OBBa_R0011 promoter which can only be activated and then expressing CAHS and mRFPBBa_J04650 after IPTG is added.
The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 375
    Illegal NheI site found at 1679
    Illegal NheI site found at 1702
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1376
    Illegal AgeI site found at 1488
  • 1000
    COMPATIBLE WITH RFC[1000]