Difference between revisions of "Part:BBa K2656013"
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Part [https://parts.igem.org/Part:BBa_I746916 BBa_I746916] standarized as Phytobrick. Super Folder Green Fluorescent Protein coding sequence. It can be used to assemble transcriptional units with the Golden Gate assembly method.
 
Part [https://parts.igem.org/Part:BBa_I746916 BBa_I746916] standarized as Phytobrick. Super Folder Green Fluorescent Protein coding sequence. It can be used to assemble transcriptional units with the Golden Gate assembly method.
  
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In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we have obtained the emission and excitation spectra in the conditions of our equipment. To do this, we have used the transcriptional unit [https://parts.igem.org/Part:BBa_K2656100 BBa_K2656100 ] assembled in a Golden Braid alpha1 plasmid'''(REF)'''. By using this protocol '''(REF)''' with the parameters of table 1, figure 1 has been obtained.
+
In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we have obtained the emission and excitation spectra in the conditions of our equipment. To do this, we have used the transcriptional unit [https://parts.igem.org/Part:BBa_K2656100 BBa_K2656100 ] assembled in a Golden Braid alpha1 plasmid'''(REF)'''. By using this protocol '''(REF)''' with the parameters of Table 1, Figure 1 has been obtained.
  
  

Revision as of 20:07, 28 September 2018


sfGFP Coding Sequence

Part BBa_I746916 standarized as Phytobrick. Super Folder Green Fluorescent Protein coding sequence. It can be used to assemble transcriptional units with the Golden Gate assembly method.

In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we have obtained the emission and excitation spectra in the conditions of our equipment. To do this, we have used the transcriptional unit BBa_K2656100 assembled in a Golden Braid alpha1 plasmid(REF). By using this protocol (REF) with the parameters of Table 1, Figure 1 has been obtained.


Parameter Value
Sample number 6
Minimum excitation wavelenght (nm) 420
Maximum excitation wavelenght (nm) 525
Emission wavelenght (nm) 545
Minimum emission wavelenght (nm) 470
Maximum emission wavelenght (nm) 580
Excitation wavelenght (nm) 450
Gain (G) 50
Table 1. Parameters used to obtain the spectra


sfGFP spectra.
Figure 1. sfGFP emission and excitation spectra


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 14