Difference between revisions of "Part:BBa K2810003"

 
 
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<partinfo>BBa_K2810003 short</partinfo>
  
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BCR3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphid bacteriocyte cells].
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This is the full coding sequence from the Myzus persisae BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi.
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BCR3_full is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].
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It is flanked by BsaI sites together 5' TACT and 3' CCAT cloning sequences for subsequent cloning into a level 1 plasmid.
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This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in [https://parts.igem.org/Part:BBa_K2810004 BBa_K2810004].
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2810003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2810003 parameters</partinfo>
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Latest revision as of 18:24, 24 August 2018

Full sequence for Myzus persisae BCR3 secreted protein

BCR3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphid bacteriocyte cells].

This is the full coding sequence from the Myzus persisae BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi.

BCR3_full is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].

It is flanked by BsaI sites together 5' TACT and 3' CCAT cloning sequences for subsequent cloning into a level 1 plasmid.

This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in BBa_K2810004.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]