Difference between revisions of "Part:BBa K2810003"

 
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BCR3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids  bacteriocyte cells].
  
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__NOTOC__
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<partinfo>BBa_K2810003 short</partinfo>
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This is the full coding sequence from the Myzus persisae BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi.
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BCR3_full is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].
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It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence
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This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in BBa_K2810004
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2810003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2810003 parameters</partinfo>
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Revision as of 18:14, 24 August 2018

BCR3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids bacteriocyte cells].


Full sequence for Myzus persisae BCR3 secreted protein

This is the full coding sequence from the Myzus persisae BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi.

BCR3_full is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].

It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in BBa_K2810004


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]