Difference between revisions of "Part:BBa K2442399"
 
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[[Image:Glasgow_rnap_gate.png|450px|thumb|center|A schematic representation of a final split-T7 RNAP AND-gate]]
 
[[Image:Glasgow_rnap_gate.png|450px|thumb|center|A schematic representation of a final split-T7 RNAP AND-gate]]
  
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This T7 RNA Polymerase split C-term coding sequence part did not exist, as it was directly PCR amplified with the B0032 ribosome binding site - this composite is [https://parts.igem.org/Part:BBa_K2442402 K2442402].
  
  
  
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===Usage and Biology===
 
===Usage and Biology===
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This nonexistent part was used for assembly of larger composite split-T7 RNAP AND-gate constructs.
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*[https://parts.igem.org/Part:BBa_K2442402 K2442402] B0032 + split-T7 RNAP C-term
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*[https://parts.igem.org/Part:BBa_K2442409 K2442409] [split T7 RNAP module 2] lacI regulated promoter + RBS + T7 rnap C-term + double terminator
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*[https://parts.igem.org/Part:BBa_K2442410 K2442410] Full split-T7 RNAP AND-gate circuit [Split RNAP module 1 + Split RNAP module 2]
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Latest revision as of 03:43, 2 November 2017


T7 RNA Polymerase split C-term


This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-T7 RNAP AND-gate


This T7 RNA Polymerase split C-term coding sequence part did not exist, as it was directly PCR amplified with the B0032 ribosome binding site - this composite is K2442402.


Usage and Biology

This nonexistent part was used for assembly of larger composite split-T7 RNAP AND-gate constructs.

  • K2442402 B0032 + split-T7 RNAP C-term
  • K2442409 [split T7 RNAP module 2] lacI regulated promoter + RBS + T7 rnap C-term + double terminator
  • K2442410 Full split-T7 RNAP AND-gate circuit [Split RNAP module 1 + Split RNAP module 2]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]