Difference between revisions of "Part:BBa K2442405"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2442405 short</partinfo>
 
<partinfo>BBa_K2442405 short</partinfo>
  
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====This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.====
  
 
[[Image:Glasgow_gfp_gate.png|450px|thumb|center|A schematic representation of a final split-GFP AND-gate]]
 
[[Image:Glasgow_gfp_gate.png|450px|thumb|center|A schematic representation of a final split-GFP AND-gate]]
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This is a composite of the GFP N-term split subunit (GFP1) [https://parts.igem.org/Part:BBa_K1789003 K1789003], the well characterised double-terminator construct [https://parts.igem.org/Part:BBa_B0015 B0015], and the well characterised tetR regulated promoter [https://parts.igem.org/Part:BBa_R0040 R0040]
 
This is a composite of the GFP N-term split subunit (GFP1) [https://parts.igem.org/Part:BBa_K1789003 K1789003], the well characterised double-terminator construct [https://parts.igem.org/Part:BBa_B0015 B0015], and the well characterised tetR regulated promoter [https://parts.igem.org/Part:BBa_R0040 R0040]
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Latest revision as of 03:27, 2 November 2017

[split GFP module 1] tetR repressible promoter + RBS + GFP N-term + double terminator

This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-GFP AND-gate


This is a composite of the GFP N-term split subunit (GFP1) K1789003, the well characterised double-terminator construct B0015, and the well characterised tetR regulated promoter R0040


Usage and Biology

this composite was used for assembly of larger composite split-GFP AND-gate constructs.

  • K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]