Difference between revisions of "Part:BBa K2442405"

Latest revision as of 03:27, 2 November 2017

[split GFP module 1] tetR repressible promoter + RBS + GFP N-term + double terminator

This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-GFP AND-gate

This is a composite of the GFP N-term split subunit (GFP1) K1789003, the well characterised double-terminator construct B0015, and the well characterised tetR regulated promoter R0040

Usage and Biology

this composite was used for assembly of larger composite split-GFP AND-gate constructs.

K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K2442405 short</partinfo>
-...
+====This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.====
+[[Image:Glasgow_gfp_gate.png|450px|thumb|center|A schematic representation of a final split-GFP AND-gate]]
+This is a composite of the GFP N-term split subunit (GFP1) [https://parts.igem.org/Part:BBa_K1789003 K1789003], the well characterised double-terminator construct [https://parts.igem.org/Part:BBa_B0015 B0015], and the well characterised tetR regulated promoter [https://parts.igem.org/Part:BBa_R0040 R0040]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+this composite was used for assembly of larger composite split-GFP AND-gate constructs.
+*[https://parts.igem.org/Part:BBa_K2442408 K2442408] Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]
 <!-- -->