Difference between revisions of "Part:BBa K2442406"

Latest revision as of 03:27, 2 November 2017

[split GFP module 2] lacI regulated promoter + RBS + GFP C-term + double terminator

This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-GFP AND-gate

This is a composite of the GFP C-term split subunit (GFP2) K2442400, the well characterised double-terminator construct B0015, and the well characterised lacI regulated promoter R0011

Usage and Biology

this composite was used for assembly of larger composite split-GFP AND-gate constructs.

K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 264

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K2442406 short</partinfo>
+====This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.====
 [[Image:Glasgow_gfp_gate.png|450px|thumb|center|A schematic representation of a final split-GFP AND-gate]]
-This is a composite of the GFP C-term split subunit (GFP2) [https://parts.igem.org/Part:BBa_K1789003 K1789003], the well characterised double-terminator construct [https://parts.igem.org/Part:BBa_B0015 B0015], and the well characterised lacI regulated promoter [https://parts.igem.org/Part:BBa_R0011 R0011]
+This is a composite of the GFP C-term split subunit (GFP2) [https://parts.igem.org/Part:BBa_K2442400 K2442400], the well characterised double-terminator construct [https://parts.igem.org/Part:BBa_B0015 B0015], and the well characterised lacI regulated promoter [https://parts.igem.org/Part:BBa_R0011 R0011]