Difference between revisions of "Part:BBa K2442405"
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This is a composite of the GFP N-term split subunit (GFP1) [https://parts.igem.org/Part:BBa_K1789003 K1789003], the well characterised double-terminator construct [https://parts.igem.org/Part:BBa_B0015 B0015], and the well characterised tetR regulated promoter [https://parts.igem.org/Part:BBa_R0040 R0040] | This is a composite of the GFP N-term split subunit (GFP1) [https://parts.igem.org/Part:BBa_K1789003 K1789003], the well characterised double-terminator construct [https://parts.igem.org/Part:BBa_B0015 B0015], and the well characterised tetR regulated promoter [https://parts.igem.org/Part:BBa_R0040 R0040] | ||
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+ | This N-term GFP part was found to contain an extra A nucleotide immediately preceding the start-codon ATG. This erroneous nucleotide is present in the parts-registry sequencing for K1789003, but is not reported as an error. Unfortunately, now both split-GFP modules might be affected by improperly spaced ribosome binding sites. Despite this we proceeded to the next assembly stage. | ||
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+ | [[Image:Glasgow_ANDgate_Figure_4.png|450px|thumb|center|A Biobrick prefix error in GFP N-term part.</b> Alignment of a sequencing read of the split GFP N-terminal to a reference sequence plasmid map. The base highlighted in red indicate an insertion has occurred at those positions relative to the reference sequence. The single-A insertion may correspond to the use a taq-polymerase during the original amplification of this coding sequence. Alignments performed in ApE.]] | ||
Revision as of 03:18, 2 November 2017
[split GFP module 1] tetR repressible promoter + RBS + GFP N-term + double terminator
This is a composite of the GFP N-term split subunit (GFP1) K1789003, the well characterised double-terminator construct B0015, and the well characterised tetR regulated promoter R0040
This N-term GFP part was found to contain an extra A nucleotide immediately preceding the start-codon ATG. This erroneous nucleotide is present in the parts-registry sequencing for K1789003, but is not reported as an error. Unfortunately, now both split-GFP modules might be affected by improperly spaced ribosome binding sites. Despite this we proceeded to the next assembly stage.
Usage and Biology
this composite was used for assembly of larger composite split-GFP AND-gate constructs.
- K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]