Difference between revisions of "Part:BBa K2213009"
 
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<partinfo>BBa_K2213009 short</partinfo>
 
<partinfo>BBa_K2213009 short</partinfo>
 
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The PduD(1-20) localisation tag, mCherry and PPK (from <i>C. glutamicim</i>) under control of the low strength Anderson promoter (https://parts.igem.org/Part:BBa_J23105). PPK is tagged with His6 (see figure 1).<br>
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The PduD(1-20) localisation tag, mCherry and PPK (from <i>C. glutamicum</i>) under control of the low strength Anderson promoter (https://parts.igem.org/Part:BBa_J23105). PPK is tagged with His6 (see figure 1).<br>
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https://static.igem.org/mediawiki/2017/0/0f/Low-tag-mcherry-PPK.png<br>
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<b>Figure 1:</b> Schematic of LowPromoter_PduD(1-20)_mCherry_cgPPK2<br>
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The Manchester iGEM team has combined this part with BBa_K2213002, which contains EutLK under the control of an araBad promoter, to make the composite part, BBa_K2213013. Coexpression of BBa_K2213013 and the other composite part, BBa_K2213012 (see: https://parts.igem.org/Part:BBa_K2213012) showed that cgPPk2 was localised in the Eut microcompartment, and served as a proof of concept for the Manchester team's project. Characterisation of BBa_K2213013 can be found at: https://parts.igem.org/Part:BBa_K2213013 and characterisation of BBa_K2213002 can be found at: https://parts.igem.org/Part:BBa_K2213002.
 
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https://static.igem.org/mediawiki/2017/0/0f/Low-tag-mcherry-PPK.png
 
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<b>Figure 1:</b> Schematic of LowPromoter_PduD(1-20)_mCherry_cgPPK2
 
 
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Latest revision as of 03:07, 2 November 2017


LowPromoter_PduD(1-20)_mCherry_cgPPK2
The PduD(1-20) localisation tag, mCherry and PPK (from C. glutamicum) under control of the low strength Anderson promoter (https://parts.igem.org/Part:BBa_J23105). PPK is tagged with His6 (see figure 1).

Low-tag-mcherry-PPK.png
Figure 1: Schematic of LowPromoter_PduD(1-20)_mCherry_cgPPK2

The Manchester iGEM team has combined this part with BBa_K2213002, which contains EutLK under the control of an araBad promoter, to make the composite part, BBa_K2213013. Coexpression of BBa_K2213013 and the other composite part, BBa_K2213012 (see: https://parts.igem.org/Part:BBa_K2213012) showed that cgPPk2 was localised in the Eut microcompartment, and served as a proof of concept for the Manchester team's project. Characterisation of BBa_K2213013 can be found at: https://parts.igem.org/Part:BBa_K2213013 and characterisation of BBa_K2213002 can be found at: https://parts.igem.org/Part:BBa_K2213002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 908
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 146
    Illegal SapI.rc site found at 1587