Difference between revisions of "Part:BBa I739002"
 
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===Part Structure===
 
===Part Structure===
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<p>The Biobrick encodes lacI ([https://parts.igem.org/wiki/index.php/Part:BBa_C0012 BBa_C0012]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of lacI is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
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<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged LacI ([https://parts.igem.org/wiki/index.php/Part:BBa_C0012 BBa_C0012]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23105 BBa_J23105] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of lacI is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
  
 
===Mode of Action===
 
===Mode of Action===
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<p>TetR binds to the pTet regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0040 BBa_R0040)] and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.</p>
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<p>LacI binds to the pLac regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0010 BBa_R0010)] (or to the PLlac01 hybrid regulator [https://parts.igem.org/wiki/index.php/Part:BBa_R0011 BBa_R0011] respectively) and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.</p>
  
 
===Purpose===
 
===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project describtion, this part is also termed ''Part 1''. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04400 BBa_Q04400].</p>
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<p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 2''. The constitutively synthesized LacI interacts with the double promoters [https://parts.igem.org/wiki/index.php/Part:BBa_I739103 BBa_I739103] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739107 BBa_I739107] which are parts of composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739016 BBa_I739016] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739011 BBa_I739011] respectively. A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04121 BBa_Q04121].</p>
  
 
===Testing===
 
===Testing===
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Pst: ok<br>
 
Pst: ok<br>
 
</p>
 
</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:49, 26 October 2007

Constitutive expression cassette for LacI +LVA (J23100.B0034.C0012.B0015)

Part Structure

The Biobrick encodes LVA-tagged LacI (BBa_C0012) under control of the constitutive promoter BBa_J23105 followed by the ribosome binding site BBa_B0034. The transcription of lacI is terminated by the double terminator BBa_B0015.

Mode of Action

LacI binds to the pLac regulator (BBa_R0010) (or to the PLlac01 hybrid regulator BBa_R0011 respectively) and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 2. The constitutively synthesized LacI interacts with the double promoters BBa_I739103 and BBa_I739107 which are parts of composites BBa_I739016 and BBa_I739011 respectively. A similar (but not used) construct in this context is BBa_Q04121.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]