Difference between revisions of "Part:BBa K2475003"
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This composite part consists of the Sup35 prion domain fused with the n-terminal fragment of the YFP complex. It does not exhibit fluorescence by itself, and must associate with the c-terminal fragment in order for fluorescence to be expressed. This was used, in conjunction with PrD-cYFP, in flow cytometry experiments to test if the aggregation domains encourage joining of the two protein halves and increases YFP expression. (Include link to other part for the registry) | This composite part consists of the Sup35 prion domain fused with the n-terminal fragment of the YFP complex. It does not exhibit fluorescence by itself, and must associate with the c-terminal fragment in order for fluorescence to be expressed. This was used, in conjunction with PrD-cYFP, in flow cytometry experiments to test if the aggregation domains encourage joining of the two protein halves and increases YFP expression. (Include link to other part for the registry) | ||
| − | Used with <partinfo> | + | Sup35 aggregation domain is from <partinfo>BBa_K2475000</partinfo>. |
| + | |||
| + | Used with <partinfo>BBa_K2475004</partinfo>. | ||
| + | <hr> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 01:18, 2 November 2017
Sup 35 Prion Domain fused to n-terminal Yellow Fluorescent Protein (PrD-nYFP)
This composite part consists of the Sup35 prion domain fused with the n-terminal fragment of the YFP complex. It does not exhibit fluorescence by itself, and must associate with the c-terminal fragment in order for fluorescence to be expressed. This was used, in conjunction with PrD-cYFP, in flow cytometry experiments to test if the aggregation domains encourage joining of the two protein halves and increases YFP expression. (Include link to other part for the registry)
Sup35 aggregation domain is from BBa_K2475000.
Used with BBa_K2475004.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 715
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]