Difference between revisions of "Part:BBa K2455002:Design"

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===Design Notes===
 
===Design Notes===
−
The first step in the insertion was amplification of [[Part:BBa_K864100|SYFP2]] using primers matching the USER Cassette of the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette]. To do this we used the following primers:
+
The first step in the insertion was amplification of [[Part:BBa_K864100|SYFP2]] using primers matching the USER Cassette of the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette]]. To do this we used the following primers:
  
 
Forward primer: CGTGCGAUCA-ATGGTTAGCAAGGGCGAAG
 
Forward primer: CGTGCGAUCA-ATGGTTAGCAAGGGCGAAG
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===Source===
 
===Source===
  
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Needed
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The biobrick is an improved version of the [https://parts.igem.org/Part:BBa_K864100|Super Yellow Fluorescent Protein 2 (SYFP2)].
  
 
===References===
 
===References===
 +
Chang, M. <i>et al.</i> (2005). Cellular Internalization of Fluorescent Proteins via Arginine-rich Intracellular Delivery Peptide in Plant Cells. <i>PCP Plant and Cell Physiology</i>, <b>46(3)</b>, pp. 482-8.
 +
 +
Eudes & Chugh (2008). Cell-penetrating peptides. <i>Plant Signal Behav.</i>, <b>3(8)</b>, pp. 549–550.

Latest revision as of 01:08, 2 November 2017


CPP-SYFP2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The first step in the insertion was amplification of SYFP2 using primers matching the USER Cassette of the Cell-Penetrating USER Cassette. To do this we used the following primers:

Forward primer: CGTGCGAUCA-ATGGTTAGCAAGGGCGAAG

Reverse primer: CACGCGAUGA-TTTATACAGCTCATCCATACCCAGGG

Subsequently the PCR product could be gel-extracted and ligated into the Cell-Penetrating USER Cassette biobrick.

Finally for the creation of a biobrick version the following set of primers were used:

Forward primer: ATCCGGAATTCGCGGCCGCTTCTAG-ATGCGTCGCCGTCGCCGTCGCCGTCGCCGTGAATGCGTGCGATC

Reverse primer: CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTA-ATGGTGATGGTGATGATGTAAG

Source

The biobrick is an improved version of the Yellow Fluorescent Protein 2 (SYFP2).

References

Chang, M. et al. (2005). Cellular Internalization of Fluorescent Proteins via Arginine-rich Intracellular Delivery Peptide in Plant Cells. PCP Plant and Cell Physiology, 46(3), pp. 482-8.

Eudes & Chugh (2008). Cell-penetrating peptides. Plant Signal Behav., 3(8), pp. 549–550.