Difference between revisions of "Part:BBa K2380002"

Latest revision as of 22:53, 1 November 2017

Chitin synthase NodC with RBS and AraC promoter system.

The Chitin Synthase (CHS) NodC from Rhizobium leguminosarum is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS (BBa_K2380024) and the expression of NodC is regulated by the AraC promoter system (BBa_K808000). For further characterizations please visit BBa_K2380000 or our [http://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase wiki].

Plasmidcard

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
Illegal AgeI site found at 1613
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

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 <partinfo>BBa_K2380002 short</partinfo>
-The [https://parts.igem.org/Part:BBa_K2380000 Chitin Synthase (CHS) NodC] from <i>Rhizobium leguminosarum</i> is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS (BBa_K2380024) and the expression of NodC is regulated by the AraC promoter system (BBa_808000).
+The [https://parts.igem.org/Part:BBa_K2380000 Chitin Synthase (CHS) NodC] from <i>Rhizobium leguminosarum</i> is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS [https://parts.igem.org/Part:BBa_K2380024 (BBa_K2380024)] and the expression of NodC is regulated by the AraC promoter system [https://parts.igem.org/Part:BBa_K808000 (BBa_K808000)]. For further characterizations please visit [https://parts.igem.org/Part:BBa_K2380000 BBa_K2380000] or our [http://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase wiki].
 <h2>Plasmidcard</h2>