Difference between revisions of "Part:BBa K2382013"

(→‎Improvemen)
(→‎Characterization of the EAAAK+ RFP+Histag)
 
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==Characterization of the EAAAK+ RFP+Histag==
 
==Characterization of the EAAAK+ RFP+Histag==
 
===Improvement===
 
===Improvement===
−
This is an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes a RFP that have
+
This is an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes a RFP that has
 
the same amino acids as BBa_E1010 and having a His-Tag at the end. <br>
 
the same amino acids as BBa_E1010 and having a His-Tag at the end. <br>
 
This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.
 
This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.

Latest revision as of 22:35, 1 November 2017

EAAAK+ RFP+Histag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 606
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.


Characterization of the EAAAK+ RFP+Histag

Improvement

This is an improvement of previous BioBrick Part BBa_E1010. It encodes a RFP that has the same amino acids as BBa_E1010 and having a His-Tag at the end.
This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.

References

BioBrick Part BBa_E1010