Difference between revisions of "Part:BBa K2446021"
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<partinfo>BBa_K2446021 short</partinfo> | <partinfo>BBa_K2446021 short</partinfo> | ||
− | + | This part is a mammalian synthetic promoter (SynPro) based on CMV promoter (<partinfo>BBa_K2446053</partinfo>) and TF binding sites . We inset the TF binding sites before the promoter. minCMV promoter is a minimized promoter from human. In this way, we can use our mammalian synthetic transcription factors (SynTFs) which is a fusing protein TF-DBD_(G4S) linker_NLS_Vp64. The TF-Vp64 can bind to the TF binding sites specificallyand the VP64 domain can active the expression of minCMV promoter [[#References|[1]]]. It can use as a "or" gate regulated by SynTF-VP64. | |
+ | [[file:T--Fudan--design.png|500px|middle|'''Figure 1''': The design of SynTF-SynPros.]] | ||
+ | <div> | ||
+ | Figure 1: The design of SynTF-SynPros. | ||
+ | </div> | ||
+ | |||
+ | But for the limitation of time, we haven’t complete the test of this group of SynPros. Another group of SynTF-KRAB&SynPros(Sv40) works well. They are all in the table below. | ||
+ | <table border="2" width="800px" > | ||
+ | <tr> | ||
+ | <th>SynTFs </th> | ||
+ | <th>SynPros</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Gal4-KRAB(TF-KRAB-1) <partinfo>BBa_K2446037</partinfo></td> | ||
+ | <td>Sv40-UAS(Sv40-UAS) <partinfo>BBa_K2446036</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_PIP_KRAB(TF-KRAB-2) <partinfo>BBa_K2446045</partinfo></td> | ||
+ | <td>SV40_2/4/8_PIP <partinfo>BBa_K2446033</partinfo>/<partinfo>BBa_K2446034</partinfo>/<partinfo>BBa_K2446035</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_21-16KRAB(TF-KRAB-3) <partinfo>BBa_K2446039 </partinfo></td> | ||
+ | <td>SV40_8_ZF_21-16 <partinfo>BBa_K2446030</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_42-10_KRAB(TF-KRAB-4) <partinfo>BBa_K2446040</partinfo></td> | ||
+ | <td>SV40_8_ZF_42-10 <partinfo>BBa_K2446025</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_43-8_KRAB(TF-KRAB-5) <partinfo>BBa_K2446041</partinfo></td> | ||
+ | <td>SV40_2/4/8_ZF_43-8 <partinfo>BBa_K2446026</partinfo>/<partinfo>BBa_K2446027</partinfo>/<partinfo>BBa_K2446028</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZF_54-8_KRAB(TF-KRAB-6) <partinfo>BBa_K2446042</partinfo></td> | ||
+ | <td>SV40_8_ZF_54-8 <partinfo>BBa_K2446029</partinfo></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>ZFHD1_KRAB(TF-KRAB-7) <partinfo>BBa_K2446043</partinfo></td> | ||
+ | <td>SV40_4_ZFHD1 <partinfo>BBa_K2446032</partinfo></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<partinfo>BBa_K2446021 parameters</partinfo> | <partinfo>BBa_K2446021 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | ===References=== | ||
+ | [1] R. R. Beerli, D. J. Segal, B. Dreier, C. F. Barbas, Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proceedings of the National Academy of Sciences 95, 14628-14633 (1998). |
Revision as of 20:56, 1 November 2017
CMV_8_ZF54-8/43-8 promoter
This part is a mammalian synthetic promoter (SynPro) based on CMV promoter (BBa_K2446053) and TF binding sites . We inset the TF binding sites before the promoter. minCMV promoter is a minimized promoter from human. In this way, we can use our mammalian synthetic transcription factors (SynTFs) which is a fusing protein TF-DBD_(G4S) linker_NLS_Vp64. The TF-Vp64 can bind to the TF binding sites specificallyand the VP64 domain can active the expression of minCMV promoter [1]. It can use as a "or" gate regulated by SynTF-VP64.
Figure 1: The design of SynTF-SynPros.
But for the limitation of time, we haven’t complete the test of this group of SynPros. Another group of SynTF-KRAB&SynPros(Sv40) works well. They are all in the table below.
SynTFs | SynPros |
---|---|
Gal4-KRAB(TF-KRAB-1) BBa_K2446037 | Sv40-UAS(Sv40-UAS) BBa_K2446036 |
ZF_PIP_KRAB(TF-KRAB-2) BBa_K2446045 | SV40_2/4/8_PIP BBa_K2446033/BBa_K2446034/BBa_K2446035 |
ZF_21-16KRAB(TF-KRAB-3) BBa_K2446039 | SV40_8_ZF_21-16 BBa_K2446030 |
ZF_42-10_KRAB(TF-KRAB-4) BBa_K2446040 | SV40_8_ZF_42-10 BBa_K2446025 |
ZF_43-8_KRAB(TF-KRAB-5) BBa_K2446041 | SV40_2/4/8_ZF_43-8 BBa_K2446026/BBa_K2446027/BBa_K2446028 |
ZF_54-8_KRAB(TF-KRAB-6) BBa_K2446042 | SV40_8_ZF_54-8 BBa_K2446029 |
ZFHD1_KRAB(TF-KRAB-7) BBa_K2446043 | SV40_4_ZFHD1 BBa_K2446032 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] R. R. Beerli, D. J. Segal, B. Dreier, C. F. Barbas, Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proceedings of the National Academy of Sciences 95, 14628-14633 (1998).