Difference between revisions of "Part:BBa K2382013"
Herlohuang (Talk | contribs) (→Characterization of the EAAAK+ RFP+Histag) |
Herlohuang (Talk | contribs) |
||
| Line 27: | Line 27: | ||
__TOC__ | __TOC__ | ||
| − | ==Improvemen== | + | |
| + | ==Characterization of the EAAAK+ RFP+Histag== | ||
| + | ===Improvemen=== | ||
This is also an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes an RFP that have | This is also an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes an RFP that have | ||
the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein | the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein | ||
with this part may have red color and the ability to be purified easily. | with this part may have red color and the ability to be purified easily. | ||
| − | |||
| − | |||
===References=== | ===References=== | ||
BioBrick Part <partinfo>BBa_E1010</partinfo> | BioBrick Part <partinfo>BBa_E1010</partinfo> | ||
Revision as of 02:04, 1 November 2017
EAAAK+ RFP+Histag
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 606
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.
Contents
Characterization of the EAAAK+ RFP+Histag
Improvemen
This is also an improvement of previous BioBrick Part BBa_E1010. It encodes an RFP that have the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein with this part may have red color and the ability to be purified easily.
References
BioBrick Part BBa_E1010