Difference between revisions of "Part:BBa K2259034"
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− | <partinfo> | + | <partinfo>BBa_K2259014 short</partinfo> |
− | + | This is a full composite part of the riboregulatory sequence Toeholed 1 [[part:BBa_K2259014]] coupled with the alpha subunit of the split antibiotic resistance gene [[Part:BBa_K2259018]]. It has a constant expression by [[part:BBa_J23101]] promoter and a double terminator at the end. | |
+ | This part is used together with [[part:BBa_K2259038]] to build a 3 or 4 plasmid SynORI selection gene circuit. Additionally, it can be used with signal activated trigger [part:BBa_K2259042]] to build a 5 plasmid SynORI selection system | ||
+ | Toehold 1 part is activated by the Trigger 1 [[part:BBa_K2259016]] | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
− | <partinfo> | + | <partinfo>BBa_K2259014 SequenceAndFeatures</partinfo> |
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===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K2259014 parameters</partinfo> |
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=Introduction= | =Introduction= | ||
==Biology== | ==Biology== | ||
− | === | + | ===The overview of 4 plasmid system==+ |
− | [[Image: | + | [[Image:4plasmid.png|center|500px|thumb|<b>Figure 1. </b> The principle scheme of SynORI 4 plasmid selection system]] |
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==Usage with SynORI (Framework for multi-plasmid systems)== | ==Usage with SynORI (Framework for multi-plasmid systems)== | ||
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SynORI is a framework for multi-plasmid systems created by ''Vilnius-Lithuania 2017'' which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]! | SynORI is a framework for multi-plasmid systems created by ''Vilnius-Lithuania 2017'' which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]! | ||
− | === | + | ===Toehold riboregulators in SynORI=== |
− | RNA | + | Toehold switches together with their corresponding RNA triggers and split antibiotic genes completes the dynamic SynORI selection system. The switches lock the translation of downstream split antibiotic genes and form an AND type gate genetic circuit which functions to stably maintain multiple plasmids in the SynORI collection. |
− | The | + | |
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+ | SynORI selection gene circuits for multi-plasmid systems: | ||
− | + | • 2 plasmids | |
− | + | Consisting of: : Two split antibiotic genes ([[part:BBa_K2259018]] and [[part:BBa_K2259019]]) | |
− | + | • 3 plasmids | |
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− | [[ | + | Consisting of: |
+ | One Toehold ([[part:BBa_K2259014]] or [[part:BBa_K2259015]]), | ||
+ | one Trigger RNA ([[part:BBa_K2259016]] or [[part:BBa_K2259017]]) and | ||
+ | split neomycin antibiotic resistance genes ([[part:BBa_K2259018]] and [[part:BBa_K2259019]]). | ||
− | + | • 4 plasmids | |
− | + | ||
+ | Consisting of: Two Toeholds ([[part:BBa_K2259014]] and [[part:BBa_K2259015]]), two Trigger RNAs ([[part:BBa_K2259016]] and [[part:BBa_K2259017]]) and split neomycin antibiotic resistance genes ([[part:BBa_K2259018]] and [[part:BBa_K2259019]]). | ||
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+ | • 5 plasmids | ||
+ | |||
+ | Consisting of: Modified phage control system [[part:BBa_K2259044]], two Toeholds ([[part:BBa_K2259014]] and [[part:BBa_K2259015]]), two repressed Trigger RNAs ([[part:BBa_K2259042]] and [[part:BBa_K2259043]]) and split neomycin antibiotic resistance genes ([[part:BBa_K2259018]] and [[part:BBa_K2259019]]). | ||
+ | |||
+ | ===Two groups of Toeholds=== | ||
+ | SynORI collection introduces two Toehold sequences termed Toehold 1 and Toehold 2 which only interact with its corresponding Trigger RNA, termed Trigger 1 and Trigger 2 and display no cross interaction. | ||
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==References== | ==References== | ||
− | + | Toehold Switches: De-Novo-Designed Regulators of Gene Expression | |
+ | |||
+ | Green, Alexander A. et al. | ||
+ | Cell, Volume 159, Issue 4, 925 - 939 |
Revision as of 21:16, 31 October 2017
Toehold 1 of SynORI selection system
This is a full composite part of the riboregulatory sequence Toeholed 1 part:BBa_K2259014 coupled with the alpha subunit of the split antibiotic resistance gene Part:BBa_K2259018. It has a constant expression by part:BBa_J23101 promoter and a double terminator at the end.
This part is used together with part:BBa_K2259038 to build a 3 or 4 plasmid SynORI selection gene circuit. Additionally, it can be used with signal activated trigger [part:BBa_K2259042]] to build a 5 plasmid SynORI selection system
Toehold 1 part is activated by the Trigger 1 part:BBa_K2259016
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Contents
Introduction
Biology
===The overview of 4 plasmid system==+
Usage with SynORI (Framework for multi-plasmid systems)
About SynORI
SynORI is a framework for multi-plasmid systems created by Vilnius-Lithuania 2017 which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!
Toehold riboregulators in SynORI
Toehold switches together with their corresponding RNA triggers and split antibiotic genes completes the dynamic SynORI selection system. The switches lock the translation of downstream split antibiotic genes and form an AND type gate genetic circuit which functions to stably maintain multiple plasmids in the SynORI collection.
SynORI selection gene circuits for multi-plasmid systems:
• 2 plasmids
Consisting of: : Two split antibiotic genes (part:BBa_K2259018 and part:BBa_K2259019)
• 3 plasmids
Consisting of: One Toehold (part:BBa_K2259014 or part:BBa_K2259015), one Trigger RNA (part:BBa_K2259016 or part:BBa_K2259017) and split neomycin antibiotic resistance genes (part:BBa_K2259018 and part:BBa_K2259019).
• 4 plasmids
Consisting of: Two Toeholds (part:BBa_K2259014 and part:BBa_K2259015), two Trigger RNAs (part:BBa_K2259016 and part:BBa_K2259017) and split neomycin antibiotic resistance genes (part:BBa_K2259018 and part:BBa_K2259019).
• 5 plasmids
Consisting of: Modified phage control system part:BBa_K2259044, two Toeholds (part:BBa_K2259014 and part:BBa_K2259015), two repressed Trigger RNAs (part:BBa_K2259042 and part:BBa_K2259043) and split neomycin antibiotic resistance genes (part:BBa_K2259018 and part:BBa_K2259019).
Two groups of Toeholds
SynORI collection introduces two Toehold sequences termed Toehold 1 and Toehold 2 which only interact with its corresponding Trigger RNA, termed Trigger 1 and Trigger 2 and display no cross interaction.
References
Toehold Switches: De-Novo-Designed Regulators of Gene Expression
Green, Alexander A. et al. Cell, Volume 159, Issue 4, 925 - 939