Difference between revisions of "Part:BBa K2110000"
 
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<partinfo>BBa_K2110000 parameters</partinfo>
 
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<partinfo>BBa_K2110000 short</partinfo>
 
* CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae.  
 
* CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae.  
  
 
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===Sequence and Features===
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<partinfo>BBa_K2110000 SequenceandFeatures</partinfo>
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<p><b>Team UiOslo Norway 2017</b> improved the documentation of this part by cloning it into pSB1C3 and sequencing it.</p>
  
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<p>>Results from PCR amplification of BBa_K2110000 inserted into pSB1C3. Primers used were <a href="https://parts.igem.org/Part:BBa_G00100">VF2</a> and <a href="https://parts.igem.org/Part:BBa_G00101">VR.</a></p>
  
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<partinfo>BBa_K2110000 SequenceandFeatures</partinfo>
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<img src="https://static.igem.org/mediawiki/2017/6/65/T--UiOslo_norway--fragmnt_cyc.jpg" width="778" height="880">
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<p><a href="https://static.igem.org/mediawiki/2017/a/a7/T--UiOslo_norway--results_cyc1_sequence_7IJ95.txt">Sequencing data of BBa_K2110000.</a> Sequencing was carried out by <a href="https://www.gatc-biotech.com/shop/en/product-overview/sanger/lightrun/lightrun-tube-barcode.html">GATC Biotech</a> and the samples sent for sequencing was prepared in accordance to the instructions in the Lightrun Sequencing protocol. Primers used were <a href="https://parts.igem.org/Part:BBa_G00100">VF2</a> and <a href="https://parts.igem.org/Part:BBa_G00101">VR,</a> as the part was still inserted into the submission vector and this made sequencing quite a bit easier.</p>
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'''Measurement'''
 
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
 

Latest revision as of 14:18, 31 October 2017


Terminator.png

chassis Saccharomyces cerevisiae, Schizosaccharmoyces Pombe

CYC1 Terminator

  • CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Team UiOslo Norway 2017 improved the documentation of this part by cloning it into pSB1C3 and sequencing it.

>Results from PCR amplification of BBa_K2110000 inserted into pSB1C3. Primers used were VF2 and VR.

Sequencing data of BBa_K2110000. Sequencing was carried out by GATC Biotech and the samples sent for sequencing was prepared in accordance to the instructions in the Lightrun Sequencing protocol. Primers used were VF2 and VR, as the part was still inserted into the submission vector and this made sequencing quite a bit easier.