Revision as of 21:41, 30 October 2017

T7 RNA Polymerase optimized for expression in E. coli

T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in Escherichia coli and contains a N-terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator.

Improved part:

Original part: <a href="https://parts.igem.org/Part:BBa_I2032" id="pageLink">BBa_I2032</a>
Submitted by: MIT
Designed by: Bartholomew Canton
Rational behind improvements: BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next vivo system we have Codon optimized for use in E. coli and attached a N-terminal hexahistidine tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21 DE3 gold cells by putting it under the control of a T7 promoter (<a href="https://parts.igem.org/Part:BBa_I719005" id="pageLink">BBa_I719005</a>), RBS (<a href="https://parts.igem.org/Part:BBa_B0034" id="pageLink">BBa_B0034</a>) and double terminator (<a href="https://parts.igem.org/Part:BBa_B0015" id="pageLink">BBa_B0015</a>). Sequence and Features