Difference between revisions of "Part:BBa K2404005"
 
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<partinfo>BBa_K2404005 short</partinfo>
 
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This is an inducible promoter that has been isolated from <i> Arabidopsis thaliana </i>. It responds to the presence of cellulose-derived oligomers in the cell, as it is a ‘Damage Associated Molecular Patterns’ (DAMP) responsive promoter. This part is suitable for Golden Gate cloning using type IIS restriction enzymes. This part is contained in the pSB1C3 plasmid.
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The [https://apps.araport.org/thalemine/report.do?id=69089924&trail=|69089924 WRKY30 promotor] is a regulatory sequence from <i>Arabidopsis thaliana</i> that has been characterised as a [http://www.plantphysiol.org/content/early/2017/02/27/pp.16.01680 ‘Damage Associated Molecular Patterns’ (DAMP)].
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This promotor is situated -653 > -168 upstream of the WRKY30 translational start point.
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This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
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This part is has been cloned into the [https://parts.igem.org/Part:BBa_P10500 P10500] plasmid using BsmBI.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2404000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2404005 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2404000 parameters</partinfo>
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<partinfo>BBa_K2404005 parameters</partinfo>
 
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Latest revision as of 21:25, 30 October 2017


WRKY30 - a promoter induced by the presence of cellulose-derived oligomers

The WRKY30 promotor is a regulatory sequence from Arabidopsis thaliana that has been characterised as a [http://www.plantphysiol.org/content/early/2017/02/27/pp.16.01680 ‘Damage Associated Molecular Patterns’ (DAMP)].

This promotor is situated -653 > -168 upstream of the WRKY30 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the P10500 plasmid using BsmBI.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 122
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 122
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 477
    Illegal BsaI.rc site found at 502