Difference between revisions of "Part:BBa K2404004"

Latest revision as of 21:23, 30 October 2017

GST6 shortened - a salicylic acid-induced promoter

The GST6 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to Salicylic acid.

This promotor includes the sequence -407 > +90 in relation to the GST6 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the P10500 plasmid using BsmBI.

The sequence includes an internal BsmBI site so was cloned into P10500 by partial digestion. This can be used for generation of level 1 plasmids but not for subsequent generation of level 2 plasmids.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 68
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 68
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 68
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 514

@@ Line 9: / Line 9: @@
 This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
-This part is has been cloned into the pSB1C3 plasmid using BsmBI.
+This part is has been cloned into the [https://parts.igem.org/Part:BBa_P10500 P10500] plasmid using BsmBI.
-The sequence includes an internal BsmBI site so was cloned into pSB1C3 by partial digestion. This can be used for generation of level 1 plasmids but not for subsequent generation of level 2 plasmids.
+The sequence includes an internal BsmBI site so was cloned into [https://parts.igem.org/Part:BBa_P10500 P10500] by partial digestion. This can be used for generation of level 1 plasmids but not for subsequent generation of level 2 plasmids.