Difference between revisions of "Part:BBa K2404004"
 
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<partinfo>BBa_K2404004 short</partinfo>
 
<partinfo>BBa_K2404004 short</partinfo>
  
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This is the most 3’ 500bp of an inducible promoter that has been isolated from <i> Arabidopsis thaliana </i>. It responds to salicylic acid. This part is suitable for Golden Gate cloning using type IIS restriction enzymes.
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The [https://apps.araport.org/thalemine/report.do?id=1010222 GST6 promotor] is a regulatory sequence from <i>Arabidopsis thaliana</i> that has been shown to respond to Salicylic acid.  
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This promotor includes the sequence -407 > +90 in relation to the GST6 translational start point.
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This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
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This part is has been cloned into the [https://parts.igem.org/Part:BBa_P10500 P10500] plasmid using BsmBI.
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The sequence includes an internal BsmBI site so was cloned into [https://parts.igem.org/Part:BBa_P10500 P10500] by partial digestion. This can be used for generation of level 1 plasmids but not for subsequent generation of level 2 plasmids.  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2404000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2404004 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2404000 parameters</partinfo>
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<partinfo>BBa_K2404004 parameters</partinfo>
 
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Latest revision as of 21:23, 30 October 2017


GST6 shortened - a salicylic acid-induced promoter

The GST6 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to Salicylic acid.

This promotor includes the sequence -407 > +90 in relation to the GST6 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the P10500 plasmid using BsmBI.

The sequence includes an internal BsmBI site so was cloned into P10500 by partial digestion. This can be used for generation of level 1 plasmids but not for subsequent generation of level 2 plasmids.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 68
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 68
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 68
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 514