Difference between revisions of "Part:BBa K2404002"

Latest revision as of 21:13, 30 October 2017

PDF1.2 shortened - a jasmonic acid-induced promoter

The PDF1.2 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to jasmonic acid.

It is a truncated version of BBa_K1467103

This promotor includes 500bp upstream of the PDF1.2 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the P10500 plasmid using BsmBI.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 408
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 517

@@ Line 11: / Line 11: @@
 This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
-This part is has been cloned into the pSB1C3 plasmid using BsmBI.
+This part is has been cloned into the [https://parts.igem.org/Part:BBa_P10500 P10500] plasmid using BsmBI.
 <!-- Add more about the biology of this part here
@@ Line 18: / Line 18: @@
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2404000 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K2404002 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K2404000 parameters</partinfo>
+<partinfo>BBa_K2404002 parameters</partinfo>
 <!-- -->