Difference between revisions of "Part:BBa K2404001:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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- We ordered the following TSH gBlock from IDT
+
- We ordered the following TSH gBlock from IDT<br>
−
tttGAATTCGCGGCCGCTTCTAGttCGTCTCtCTCAaatgACTGCTCTGTTTCTGATGAGTATGCTGTTCGGACTGGCTTGCGGGCAGGCTATGTCTTTCTGTATCCCCACTGAGTACACCATGCACATCGAGAGGAGAGAATGCGCATACTGTCTGACAATCAACACCACAATTTGCGCCGGGTATTGTATGACTAGGGACATTAATGGCAAGCTGTTCCTGCCAAAATACGCACTGTCCCAGGACGTGTGCACTTACCGGGACTTCATCTACCGCACCGTCGAGATTCCCGGATGTCCTCTGCACGTGGCCCCTTACTTCAGTTATCCAGTCGCTCTGTCATGCAAGTGTGGGAAATGCAACACTGACTATTCTGATTGTATCCATGAAGCTATTAAGACTAACTACTGCACCAAGCCTCAGAAAAGCTATCTGGTGGGCTTTTCCGTCAGCTCCTCTAGTAAGGCCCCCCCTCCATCACTGCCCTCACCTAGCAGACTGCCAGGACCCAGCGACACCCCAATCCTGCCCCAGGCTCCTGACGTGCAGGATTGCCCCGAGTGTACACTGCAGGAAAACCCTTTCTTTAGTCAGCCAGGCGCTCCCATTCTGCAGTGTATGGGATGCTGTTTCTCTCGAGCATACCCTACCCCACTGAGGAGTAAGAAAACAATGCTGGTGCAGAAGGATGTCACATCCGAGTCTACTTGCTGCGTGGCCAAAAGCTATAATCGGGTGACAGTCATGGGCGGATTCAAAGTCGAAGACCACACCGCCTGCCACTGCTCCACCTGCTACTACCATAAAAGTCTGGTTCCGCGTGGATCCCAcCATCAcCAcCATCATTGAgcttCGAGaGAGACGaaTACTAGTAGCGGCCGCTGCAGttt
+
https://static.igem.org/mediawiki/parts/2/2d/T--Cardiff_Wales--TSH_gblock.png
  
−
- This was introduced into pSB1C3 by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning
+
- This was introduced into [https://parts.igem.org/Part:BBa_P10500 BBa_P10500] by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning
  
 
===Source===
 
===Source===
  
−
This modified protein was discovered in a paper by Fares <i> et al </i> in 2001, but without the His-tags.
+
This modified protein was first used in [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558] but without the His-tags.
 
It was ordered as a g-block.
 
It was ordered as a g-block.
  
 
===References===
 
===References===

Latest revision as of 21:00, 30 October 2017


A Thyroid Stimulating Hormone antagonist with His-Tags


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 795
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 827


Design Notes

We generated this part through the following steps:


- We ordered the following TSH gBlock from IDT
T--Cardiff_Wales--TSH_gblock.png

- This was introduced into BBa_P10500 by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning

Source

This modified protein was first used in [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558] but without the His-tags. It was ordered as a g-block.

References