Difference between revisions of "Part:BBa K2374003"

Revision as of 11:23, 30 October 2017

ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)

An upstream activating sequence or upstream activation sequence (UAS) is a cis-acting regulatory sequence. It is distinct from the promoter and increases the expression of a neighbouring gene. Due to its essential role in activating transcription, the UAS is often considered to be analogous to the function of the enhancer in multicellular eukaryotes. Upstream activation sequences are a crucial part of induction, enhancing the expression of the protein of interest through increased transcriptional activity. The UAS is found adjacently upstream to a minimal promoter (TATA box) and serves as a binding site for transactivators. If the transcriptional transactivator does not bind to the UAS in the proper orientation then transcription cannot begin

Gene ple encodes Tyrosine 3-monooxygenase which also known as TH (EC:1.14.16.2). It plays an important role in the physiology of adrenergic neurons. This protein is involved in step 1 of the subpathway that synthesizes dopamine from L-tyrosine. Dopamine has critical roles in system development. Proteins known to be involved in the 2 steps of the subpathway in this organism are:
1.Tyrosine 3-monooxygenase (ple)
2.no protein annotated in this organism
This subpathway is part of the pathway dopamine biosynthesis, which is itself part of Catecholamine biosynthesis. It belongs to the biopterin-dependent aromatic amino acid hydroxylase family. We also provide ple promoter in BBa_K2374001.

We construct pSB1C3-UAS-TH and pUAST-UAS-TH. The pSB1C3-UAS-TH is for submission. Then pUAST-UAS-TH is used to do micro-injection into the D.melanogaster. Also with the other two plasmids: pUAST-ple-GAL4 (BBa_K2374005)and pUAST-ple-GAL80ts (BBa_K2374006). We must combine the three pathways to determine if the system work well. The result of our testing on D.melanogaster(pUAST-UAS-TH) is displayed below.

Sequence and Features

Functional Parameters

TH is involved in step 1 of the subpathway that synthesizes dopamine from L-tyrosine and plays an important role in the physiology of adrenergic neurons.

References

1. Webster Nocholas, Jin Jiarui, Green Stephen, Hollis Melvyn, Chambon Pierre (1988). The Yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans-activator. Cell. 52 (2): 169–178.
2. West Jr. Robert W., Yocum R. Rogers, Ptashne Mark (1984). Saccharomyces cerevisiae GAL1-GAL10 Divergenet Promoter Region: Location and Function of the Upstream Activating Sequence UAS. Molecular and Cellular Biology. 4 (11): 2467–2478.
3. Lewandoski Mark (2001). Conditional control of gene expression in the mouse. Nature Reviews Genetics. 2: 743–755.