Difference between revisions of "Part:BBa K2374003"
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    <source src="https://static.igem.org/mediawiki/2015/2/2a/China-Tongji-Project-video4-pmyo2-chR2.mp4" type="video/mp4" />pmyo2-chR2.mp4
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Revision as of 16:17, 29 October 2017


ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)

UAS-TH
Use in D.melanogaster
RFC standard RFC 10 compatible
Backbone pSB1C3
Submitted by [http://2017.igem.org/Team:Tongji_China Tongji_China 2017]


<video controls width="700"> 

   <source src="https://static.igem.org/mediawiki/2015/2/2a/China-Tongji-Project-video4-pmyo2-chR2.mp4" type="video/mp4" />pmyo2-chR2.mp4

</video>


Pale is a tyrosine hydroxylase, the first and rate-limiting step in the synthesis of dopamine (and eventually, melanin). Dopamine has critical roles in system development. We also provide Pale promoter in BBa_K2374001.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1157
    Illegal XhoI site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 535
    Illegal BsaI site found at 1501
    Illegal BsaI.rc site found at 253
    Illegal BsaI.rc site found at 1019
    Illegal BsaI.rc site found at 1168


Functional Parameters

TH is involved in step 1 of the subpathway that synthesizes dopamine from L-tyrosine and plays an important role in the physiology of adrenergic neurons.