Difference between revisions of "Part:pSB3K1"

 
 
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__NOTOC__
 
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<partinfo>BBa_I739204 short</partinfo>
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<partinfo>pSB3K1 short</partinfo>
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Plasmid pACYC177BB1 (Fig. 1) is based on pACYC177 (Fig. 2), a low-copy cloning vector (10-12 copies per cell) that confers ampicillin (ApR) and  kanamycin (KmR) resistance and harbours the p15A origin of replication.<br>
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[[Image:MapI739204.jpg|left|thumb|'''Fig. 1:''' Map of the pACYC177BB1 plasmid|250px]][[Image: Mappacyc177.jpg |right|thumb|'''Fig. 2:''' Map of the original pACYC177 plasmid|250px]]
  
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In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], the original pACYC177 was digested at its BamHI and PstI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. However, this leads to a loss of the original ampicillin resistance and only kanamycin resistance is left. The term "BB1" in pACYC177BB1 refers to "BioBrick version 1".
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<br>
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===Purpose===
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<p>This plasmid was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: [https://parts.igem.org/wiki/index.php/Part:BBa_I739006 ''Part 6''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739007  ''Part 7''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739010 ''Part 10''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739011 ''Part 11''] and the composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739018 BBa_I739018], [https://parts.igem.org/wiki/index.php/Part:BBa_I739019 BBa_I739019] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739020 BBa_I739020]. More information on the plasmid and the used assembly strategy can be found [http://2007.igem.org?title=ETHZ/Biology/Lab here].
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</p><br>
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_I739204 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>pSB3K1 parameters</partinfo>
 
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Latest revision as of 09:07, 25 October 2007

pACYC177 converted into BioBrick vector (formerly BBa_I739202)

Plasmid pACYC177BB1 (Fig. 1) is based on pACYC177 (Fig. 2), a low-copy cloning vector (10-12 copies per cell) that confers ampicillin (ApR) and kanamycin (KmR) resistance and harbours the p15A origin of replication.

Fig. 1: Map of the pACYC177BB1 plasmid
Fig. 2: Map of the original pACYC177 plasmid

In order to make this plasmid compatible with the BioBrick Standard assembly process, the original pACYC177 was digested at its BamHI and PstI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. However, this leads to a loss of the original ampicillin resistance and only kanamycin resistance is left. The term "BB1" in pACYC177BB1 refers to "BioBrick version 1".

Purpose

This plasmid was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: Part 6, Part 7, Part 10, Part 11 and the composites BBa_I739018, BBa_I739019 and BBa_I739020. More information on the plasmid and the used assembly strategy can be found [http://2007.igem.org?title=ETHZ/Biology/Lab here].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3043
    Illegal NheI site found at 1305
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3049
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3043
    Illegal BamHI site found at 3037
    Illegal XhoI site found at 1669
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3043
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3043
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 895
    Illegal AgeI site found at 1219
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 190