Difference between revisions of "Part:BBa K313002"

Latest revision as of 08:43, 27 October 2017

flpe DNA recombinase

Flpe is a site-specific DNA recombinase that recognizes FRT sequence. Flpe was derived from yeast site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))

We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Improvements

• FLPe + NLS: BBa_K2294000

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 504
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K313002 short</partinfo>
-Flpe is a site-specific DNA recombinase that that recognizes FRT sequence.
+Flpe is a site-specific DNA recombinase that recognizes FRT sequence.
-Flpe was derived from yeaset site-specific DNA recombinase Flp.
+Flpe was derived from yeast site-specific DNA recombinase Flp.
 (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))
-It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.
+We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.
+Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.<br/>
+====Improvements====
+• FLPe + NLS: <html><a href="https://parts.igem.org/Part:BBa_K2294000" target="_blank">BBa_K2294000</a></html>
 <!-- Add more about the biology of this part here