Difference between revisions of "Part:BBa K313002"
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| − | • | + | • FLPe + NLS: <html><a href="https://parts.igem.org/Part:BBa_K2294000" target="_blank">BBa_K2294000</a> |
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Revision as of 08:42, 27 October 2017
flpe DNA recombinase
Flpe is a site-specific DNA recombinase that recognizes FRT sequence. Flpe was derived from yeast site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))
We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.
Improvements
• FLPe + NLS: BBa_K2294000
Sequence and Features