Difference between revisions of "Part:BBa K2332316"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2332316 short</partinfo>
 
<partinfo>BBa_K2332316 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|Plasma Membrane Delivery System (PMDS)
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|-
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|'''Function'''
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|Delivery of protein (e.g. mCherry) to the PM
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''Abstraction Hierarchy'''
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|Part
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC10], [https://parts.igem.org/Help:Standards/Assembly/RFC12 RFC12], [https://parts.igem.org/Help:Assembly_standard_23 RFC23] & [https://parts.igem.org/Help:Assembly_standard_25 RFC25] compatible
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2017.igem.org/Team:UCL UCL iGEM 2017]
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|}
  
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LACE system
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This gene encodes nuclease deactivated Cas9 (dCas9) fused to the transactivation domain of NFκB, called ReLa p65. This protein allows targeted expression of endogenous and exogenous expression of genes via association of co-translated gRNA's.
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<html>
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</p>
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</html>
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__TOC__
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K2332316 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2332316 SequenceAndFeatures</partinfo>
  
  
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2332316 parameters</partinfo>
 
<partinfo>BBa_K2332316 parameters</partinfo>
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<!-- -->
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Revision as of 23:52, 22 October 2017


dCas9-p65 (TF)

Plasma Membrane Delivery System (PMDS)
Function Delivery of protein (e.g. mCherry) to the PM
Use in Mammalian cells
Abstraction Hierarchy Part
RFC standard RFC10, RFC12, RFC23 & RFC25 compatible
Backbone pSB1C3
Submitted by [http://2017.igem.org/Team:UCL UCL iGEM 2017]

This gene encodes nuclease deactivated Cas9 (dCas9) fused to the transactivation domain of NFκB, called ReLa p65. This protein allows targeted expression of endogenous and exogenous expression of genes via association of co-translated gRNA's.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5011
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

=