Difference between revisions of "Part:BBa I739021"
Stefan Luzi (Talk | contribs) |
Stefan Luzi (Talk | contribs) |
||
| Line 9: | Line 9: | ||
===Purpose=== | ===Purpose=== | ||
| − | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is a composite biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. ... | + | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is a composite biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. ...</p> |
===Testing=== | ===Testing=== | ||
Revision as of 12:14, 19 October 2007
Single regulated polycistronic P22 cII +LVA / EYFP +LVA expression cassette
Part Structure
The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) and LVA-tagged EYFP (BBa_E0032) under control of the double promoter BBa_I739101 followed by the ribosome binding site BBa_B0034. The transcription of P22 cII and EYFP is terminated by the double terminator BBa_B0015.
Mode of Action
P22 cII and EYFP production can be repressed by tetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is a composite biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. ...
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 275