Difference between revisions of "Part:BBa K2120304"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2120304 short</partinfo>
 
<partinfo>BBa_K2120304 short</partinfo>
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'''<Description>'''
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<br>This part is composed of two elements:
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<br>(1) Arabinose induced promoter pBAD and its inhibitor ''araC'' [https://parts.igem.org/Part:BBa_K808000 BBa_K808000].This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.
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<br>(2) Galactose induced promoter pLac's inhibitor gene ''lacIq''.
  
  

Latest revision as of 17:07, 22 October 2016


araC-pBAD+B0034+LacIq+B0015

<Description>
This part is composed of two elements:
(1) Arabinose induced promoter pBAD and its inhibitor araC BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.
(2) Galactose induced promoter pLac's inhibitor gene lacIq.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961