Difference between revisions of "Part:BBa K1919233"
Line 7: | Line 7: | ||
[[File:AarC.png]] | [[File:AarC.png]] | ||
− | + | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
<!-- --> | <!-- --> |
Revision as of 12:46, 19 October 2016
AarC coding sequence
Succinyl-CoA:Acetate CoA-Transferase(AarC) can let acetate and Succinyl-CoA become acetyl-CoA and succinate. E.coli can use acetate as energy sources by this enzyme. This enzyme also can give E.coli some ability of anti acetic acid. We made five devices for constitutive expressing AarC enzyme. These devices have different promoter. From strong to weak are BBa_K1919200 (J23100), BBa_K1919204 (J23104), BBa_K1919206 (J23106), BBa_K1919205 (J23105) and BBa_K1919214 (J23114). All of these expression devices have His-tag at C-terminal.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 106
Illegal AgeI site found at 202
Illegal AgeI site found at 214 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 52
Illegal BsaI.rc site found at 316