Device

Part:BBa_K1919204

Designed by: Haoyu Wang   Group: iGEM16_SCU-China   (2016-10-14)


J23104 promoter-Constitutive AarC Expression Device

This device can express AarC with His-tag. Promoter is BBa_J23104. His-tag and T7 terminator come from pET-23 vector. His-tag and T7 terminator come from pET-23 vector. About AarC, please see BBa_K1919233 (AarC coding sequence) page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 168
    Illegal AgeI site found at 264
    Illegal AgeI site found at 276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114
    Illegal BsaI.rc site found at 378


[edit]
Categories
Parameters
None