Difference between revisions of "Part:BBa K1919233"

Revision as of 12:35, 19 October 2016

AarC coding sequence

Succinyl-CoA:Acetate CoA-Transferase(AarC) can let acetate and Succinyl-CoA become acetyl-CoA and succinate. E.coli can use acetate as energy sources by this enzyme. This enzyme also can give E.coli some ability of anti acetic acid. We made five devices for constitutive expressing AarC enzyme. These devices have different promoter. From strong to weak are BBa_K1919200 (J23100), BBa_K1919204 (J23104), BBa_K1919206 (J23106), BBa_K1919205 (J23105) and BBa_K1919214 (J23114). All of these expression devices have His-tag at C-terminal.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 106
Illegal AgeI site found at 202
Illegal AgeI site found at 214
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 52
Illegal BsaI.rc site found at 316

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1919233 short</partinfo>
-Succinyl-CoA:Acetate CoA-Transferase(AarC) can let acetate and Succinyl-CoA become acetyl-CoA and succinate. E.coli can use acetate as energy sources by this enzyme. This enzyme also can give E.coli some ability of anti acetic acid. We made five devices for constitutive expressing AarC enzyme. These devices have different promoter. From strong to weak are <partinfo>BBa_K1919200</partinfo> (J23100), <partinfo>BBa_K1919204</partinfo> (J23104), <partinfo>BBa_K1919206</partinfo> (J23106), <partinfo>BBa_K1919205</partinfo> (J23105) and <partinfo>BBa_K1919214</partinfo> (J23114).
+Succinyl-CoA:Acetate CoA-Transferase(AarC) can let acetate and Succinyl-CoA become acetyl-CoA and succinate. E.coli can use acetate as energy sources by this enzyme. This enzyme also can give E.coli some ability of anti acetic acid. We made five devices for constitutive expressing AarC enzyme. These devices have different promoter. From strong to weak are <partinfo>BBa_K1919200</partinfo> (J23100), <partinfo>BBa_K1919204</partinfo> (J23104), <partinfo>BBa_K1919206</partinfo> (J23106), <partinfo>BBa_K1919205</partinfo> (J23105) and <partinfo>BBa_K1919214</partinfo> (J23114). All of these expression devices have His-tag at C-terminal.
 [[File:AarC.png]]