Difference between revisions of "Part:BBa K1897020"

Revision as of 18:27, 17 October 2016

p70-32-ALR-Terminator

The construct contains lldRO1-J23117-lldRO2 promoter designed by team ETH-Zurich 2015 (Part:BBa_K1847008) with a medium RBS flanked by non-coding sequences (Part:BBa_K1897032), followed by a coding sequence of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 78
Illegal NheI site found at 101
Illegal NheI site found at 501
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 425
Illegal BamHI site found at 1127
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 543
Illegal AgeI site found at 843
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 300

Revision as of 18:26, 17 October 2016 (view source) Phuongnguyen (Talk \| contribs) ← Older edit		Revision as of 18:27, 17 October 2016 (view source) Phuongnguyen (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K1897020 short</partinfo>		<partinfo>BBa_K1897020 short</partinfo>

−	The construct contains lldRO1-J23117-lldRO2 promoter designed by team ETH-Zurich 2015 ([[Part:BBa_K1847008]]) with a medium RBS flanked by non-coding sequences ([[Part:BBa_K1897032]]), followed by a ~~CDS~~ of ALR ([[Part:BBa_K1172901]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]).	+	The construct contains lldRO1-J23117-lldRO2 promoter designed by team ETH-Zurich 2015 ([[Part:BBa_K1847008]]) with a medium RBS flanked by non-coding sequences ([[Part:BBa_K1897032]]), followed by a coding sequence of ALR ([[Part:BBa_K1172901]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]).