Difference between revisions of "Part:BBa B0032"

Revision as of 21:43, 16 October 2016

RBS.3 (medium) -- derivative of BBa_0030

Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0030, BBa_B0031, BBa_B0033.

Usage and Biology

IIT Madras 2016's Characterization

Characterization of popular BioBrick RBSs

Modelling

Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts modelling]

Experimentation

Biobrick RBSs B0030, B0031, B0032, B0034 were used in our 'Noise in Device' experiment to understand the role of RBS parts in giving rise to noise.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

biology	-NA-
efficiency	0.3

(Relative to BBa_B0034)

Team Warsaw 2010's measurement

RBS strength (relative to B0034): 33,96%

@@ Line 12: / Line 12: @@
 <p>Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts modelling]</p>
 == Experimentation ==
-<p>Biobrick RBSs [https://parts.igem.org/Part:BBa_K1956022 B0030], [https://parts.igem.org/Part:BBa_K1956023 B0031], [https://parts.igem.org/Part:BBa_K1956014 B0032], [https://parts.igem.org/Part:BBa_K1956015 B0034] were used in our 'Noise in Device' experiment to understand the role of RBS parts in noise.</p>
+<p>Biobrick RBSs [https://parts.igem.org/Part:BBa_K1956022 B0030], [https://parts.igem.org/Part:BBa_K1956023 B0031], [https://parts.igem.org/Part:BBa_K1956014 B0032], [https://parts.igem.org/Part:BBa_K1956015 B0034] were used in our 'Noise in Device' experiment to understand the role of RBS parts in giving rise to noise.</p>
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