Difference between revisions of "Part:BBa B0030"
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Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts | modelling]  
 
Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts | modelling]  
 
== Experimetnation ==
 
== Experimetnation ==
−
Biobrick RBSs [https://parts.igem.org/Part:BBa_K1956022 | B0030], [https://parts.igem.org/Part:BBa_K1956023 | B0031], [https://parts.igem.org/Part:BBa_K1956014 | B0032], [https://parts.igem.org/Part:BBa_K1956015 | B0034] RBSs were used in our 'Noise in Device' experiment to understand the role of RBS parts in noise.
+
Biobrick RBSs [https://parts.igem.org/Part:BBa_K1956022 B0030], [https://parts.igem.org/Part:BBa_K1956023 B0031], [https://parts.igem.org/Part:BBa_K1956014 B0032], [https://parts.igem.org/Part:BBa_K1956015 B0034] RBSs were used in our 'Noise in Device' experiment to understand the role of RBS parts in noise.
  
 
Note: not compatible with R0053 promoter due to likely transcript secondary structure  This combination yielded very low gfp expression (see BBa_I7108).  [jcb 8/3/05].
 
Note: not compatible with R0053 promoter due to likely transcript secondary structure  This combination yielded very low gfp expression (see BBa_I7108).  [jcb 8/3/05].

Revision as of 21:35, 16 October 2016

RBS.1 (strong) -- modified from R. Weiss

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034.


Usage and Biology

IIT Madras 2016's Characterization

Characterization of popular BioBrick RBSs

Modelling

Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts | modelling]

Experimetnation

Biobrick RBSs B0030, B0031, B0032, B0034 RBSs were used in our 'Noise in Device' experiment to understand the role of RBS parts in noise.

Note: not compatible with R0053 promoter due to likely transcript secondary structure This combination yielded very low gfp expression (see BBa_I7108). [jcb 8/3/05].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Team Warsaw 2010's measurement

RBS strength (relative to B0034): 91,84%

Functional Parameters

biology-NA-
efficiency0.6
(Relative to BBa_B0034)