Difference between revisions of "Part:BBa K1897023"

 
Line 3: Line 3:
 
<partinfo>BBa_K1897023 short</partinfo>
 
<partinfo>BBa_K1897023 short</partinfo>
  
−
The construct contains a wild-type lldPRD operon promoter from E. coli (BBa_K822000) with a medium RBS (BBa_B0032), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator.
+
The construct contains a wild-type lldPRD operon promoter from E. coli ([[Part:BBa_K822000]])(BBa_K822000) with a medium RBS ([[Part:BBa_K1847008]])(BBa_B0032), followed by a CDS of ALR ([[Part:BBa_K1172901]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:27, 14 October 2016


p62-32-ALR-Terminator

The construct contains a wild-type lldPRD operon promoter from E. coli (Part:BBa_K822000)(BBa_K822000) with a medium RBS (Part:BBa_K1847008)(BBa_B0032), followed by a CDS of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 501
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 425
    Illegal BamHI site found at 1127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 543
    Illegal AgeI site found at 843
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 300